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Hospital-approved mild disinfectants are used for chamber cleaning. Alcohol and ammonia-based cleaners are avoided due to flammability and material damage risks, while steam sterilization is unsafe for acrylic and seals.

Decompression stops permit controlled release of nitrogen dissolved in tissues, preventing bubble formation. Without these pauses, rapid ascent leads to decompression sickness.

ASME PVHO-1 governs structural design, testing, and certification of pressure vessels for human occupancy, including hyperbaric chambers. NFPA covers fire safety, OSHA workplace standards, and UHMS clinical guidelines.

UHMS guidelines approve HBO for Wagner grade III diabetic ulcers (deep tissue involvement with abscess, osteomyelitis, or gangrene) that fail standard wound care. Stage I pressure ulcers and minor wounds are not approved.

Oxygen under pressure reacts violently with hydrocarbons such as oil or grease, causing ignition or explosion. All oxygen fittings must remain oil- and grease-free for safety.